Channel: The Thought Emporium

Category: Science & Technology

Tags: geneticchemistryuvhow tobiologybacteriabl21microbiologyheat shockengineeringnordvpntransformationgenetic engineeringfluorescentprotocolgeneticsdnaculturesciencephysicstutorialmicrobeglow

Description: For NordVPN’s 9th birthday, every purchase of a 2-year plan will get you 1 additional month free AND a surprise gift. Go to nordvpn.com/thoughtemporium and use code thoughtemporium! ________________________________________________________________________ BL21 E. coli are by far the most obnoxious bacteria genetic engineers usually work with for 1 simple reason. They are maddeningly difficult to modify and load with new DNA. This wouldn't be an issue if they weren't also one of the best types for producing proteins en mass, and as such have application in everything from pharmaceutical production, to producing ingredients for foods. But after a few years of tinkering with it I finally found a protocol that reliably fixes this irritating issue, and makes it easy to transform them reliably. Written protocol - github.com/thethoughtemporium/Protocols/blob/main/Transformations/bl21%20transformation.md Sebastian's spinner - tinkercad.com/things/5t5GqlGkK1i Previous videos; Modifying Bacteria - youtu.be/F0_q-fD_lyU Modifying yeast - youtu.be/DHNPnO5UOYQ Making a heat block/incubator - youtu.be/Zm378x6zJYc Growing spider silk -youtu.be/2hf9yN-oBV4 ___________________________________________________________________________ Support the show and future projects: Patreon: patreon.com/thethoughtemporium Ko-Fi: ko-fi.com/thoughtemporium Become a member: youtube.com/channel/UCV5vCi3jPJdURZwAOO_FNfQ/join _________________________________________________________________ My Social Media Pages: Instagram: instagram.com/thethoughtemporium Facebook: facebook.com/thethoughtemporium Twitter: twitter.com/emporiumthought Website: thethoughtemporium.com